Zinc Finger Proteins

Part 1. Basic Biology and Engineering of ZFPs.- PRDM14, a zinc finger protein, regulates cancer stemness.- Computational methods for analysis of the DNA-binding preferences of Cys2His2 zinc finger proteins.- Design and application of mA-specific Zinc finger proteins for the readout of DNA methylation.- Enhanced manipulation of human mitochondrial DNA heteroplasmy in vitro using tunable mtZFN technology.- Engineering RNA-binding proteins by modular assembly of RanBP2-type Zinc Fingers.- Design of a system for monitoring ubiquitination activities of E2 enzymes using engineered RING finger proteins.- Directed evolution of targeted recombinases for genome engineering.- Optical recording of cellular zinc dynamics with zinc finger based biosensors.- Delivery of superoxide dismutase using Cys2-His2 zinc-finger proteins.- Genome editing of MSCs as a platform for cell therapy.- Part 2. Methods for the Evaluation and Prevention of ZFN Cytotoxicity.- Integrated multimodal evaluation of genotoxicity in ZFN-modified primary human cells.- Selection and characterization of DNA aptamers against FokI nuclease domain.- An improved genome engineering method using surrogate reporter-coupled suicidal ZFNs.- Part 3. Delivery Methods of ZFPs.- Non-transgenic approach to deliver ZFNs in seeds for targeted genome engineering.- Gene editing in channel catfish via double electroporation of zinc finger nucleases.- Delivery of mtZFNs into early mouse embryos.- Stereotaxic surgery and viral-delivery of zinc finger epigenetic editing tools in rodent brain.- In-vivo applications of cell-penetrating zinc finger transcription factors.- Manufacturing and delivering genome-editing proteins.