Yeast Protocols
(Sprache: Englisch)
Yeast Protocols, Third Edition presents up-to-date advances in research using yeasts as models. Chapters cover topics such as basic protocols in yeast culture and genomic manipulation, protocols that study certain organelles such as mitochondria and...
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Yeast Protocols, Third Edition presents up-to-date advances in research using yeasts as models. Chapters cover topics such as basic protocols in yeast culture and genomic manipulation, protocols that study certain organelles such as mitochondria and peroxisomes and their functions in autophagy and assays commonly used in yeast-based studies that can be adapted to other organisms. As the first sequenced living organism, budding yeast S. cerevisiae and other model yeasts have helped greatly in life science research. The easy switch between the haploid and diploid state makes yeast a paradigm of genetic manipulation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Yeast Protocols, Third Edition seeks to serve both professionals and novices with newly-developed protocols to study this essential model organism.
Inhaltsverzeichnis zu „Yeast Protocols “
1. Basic Investigations in Saccharomyces cerevisiaeBrendan Curran and Virginia Bugeja
2. Isolation of Yeast Nucleic Acids
Michael Biss, Michelle Hanna and Wei Xiao
3. Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry
Ting Wei and Heping Dai
4. Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
R. Daniel Gietz
5. Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe
Johannes H. Hegemann, Sven Boris Heick, Jennifer Pöhlmann, Markus Langen and Ursula Fleig
6. See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry
Changhui Deng and Andrew N. Krutchinsky
7. Yeast Two Hybrid Liquid Screening
Elisa Donnard, Erica M. Queiroz, J. Miguel Ortega, and R. Daniel Gietz
8. Targeted Mutagenesis of a Specific Gene in Yeast
Likui Zhang, Alina Radziwon, and Linda J. Reha-Krantz
9. Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae
Kai Stefan Dimmer
10. Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy
Wei-Pang Huang, Takahiro Shintani, and Zhiping Xie
11. Assays for Autophagy II: Mitochondrial Autophagy
Tomotake Kanki and Koji Okamoto
12. Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction
Danielle Hagstrom and Changle Ma
13. Yeast Survival and Growth Assays
Xin Xu, Amanda Lambrecht, and Wei Xiao
14. Spontaneous Mutagenesis Assay
Susan Blackwell, Michelle Hanna, and Wei Xiao
15. Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene
Bernard A. Kunz
16. piggyBac Transposon-Based Insertional Mutagenesis for the Fission
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Yeast Schizosaccharomyces pombe
Jun Li and Li-Lin Du
17. Replicative and Chronological Life Span Assays
Spike D. L. Postnikoff and Troy A. A. Harkness
18. Detection of Protein Arginine Methylation in Saccharomyces cerevisiae
Christopher Aa. Jackson and Michael C. Yu
19. Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae
Lindsay G. Ball and Wei Xiao
20. Ligand Engineering Using Yeast Surface Display
Benjamin J. Hackel
21. Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay
Kris Barreto and C. Ronald Geyer
Jun Li and Li-Lin Du
17. Replicative and Chronological Life Span Assays
Spike D. L. Postnikoff and Troy A. A. Harkness
18. Detection of Protein Arginine Methylation in Saccharomyces cerevisiae
Christopher Aa. Jackson and Michael C. Yu
19. Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae
Lindsay G. Ball and Wei Xiao
20. Ligand Engineering Using Yeast Surface Display
Benjamin J. Hackel
21. Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay
Kris Barreto and C. Ronald Geyer
... weniger
Bibliographische Angaben
- 2014, 3. Aufl., 313 Seiten, 27 farbige Abbildungen, Maße: 18,5 x 26,1 cm, Gebunden, Englisch
- Herausgegeben: Wei Xiao
- Verlag: Springer, Berlin
- ISBN-10: 1493907980
- ISBN-13: 9781493907984
- Erscheinungsdatum: 20.05.2014
Sprache:
Englisch
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