Xenopus Protocols

Xenopus tropicalis as a Model Organism for Genetics and Genomics: Past, Present and Future.-Husbandry of Xenopus tropicalis.-Generating Diploid Embryos from Xenopus tropicalis.-Navigating the Xenopus tropicalis Genome.-Genetic Analysis of Xenopus tropicalis.-Forward Genetic Screens in Xenopus using Transposon-mediated Insertional Mutagenesis.-Targeted Gene Disruption with Engineered Zinc-finger Nucleases (ZFNs).-Reverse Genetic Studies using Antisense Morpholino Oligonucleotides.-Chemical Genetics and Drug Discovery in Xenopus.-Maternal mRNA Knock-down Studies: Antisense Experiments using the Host-transfer Technique in Xenopus laevis and Xenopus tropicalis.-Generating Transgenic Frog Embryos by Restriction Enzyme Mediated Integration (REMI).-A Simple Method of Transgenesis using I-SceI meganuclease in Xenopus.-Using FC31 Integrase to Mediate Insertion of DNA in Xenopus Embryos.-Xenopus Transgenics: Methods using Transposons.-Comparative Genomics-based Identification and Analysis of Cis-regulatory Elements.-Tet-On Binary Systems for Tissue-specific and Inducible Transgene Expression.-Chromatin Immunoprecipitation Analysis of Xenopus Embryos.-Transcriptomics Using Next Generation Sequencing Technologies.-Databases of Gene Expression in Xenopus Development.-Investigating Alternative RNA Splicing in Xenopus.-Immunoisolation of Protein Complexes from Xenopus.-Complementary Proteomic Analysis of Protein Complexes.-Antibody Development and use in Chromogenic and Fluorescent Immunostaining.-Multiple fluorescent in situ mRNA Hybridization (FISH) on Whole Mounts and Sections.-Methods to Analyze microRNA Expression and Function During Xenopus Development.-A Bromodeoxyuridine (BrdU) Based Protocol for Characterizing Proliferating Progenitors in Xenopus Embryos.-Microscopy Tools for Quantifying Developmental Dynamics in XenopusEmbryos.-Mathematical Modelling of Gene RegulatoryNetworks in Xenopus Development.-Stem-cell-like Embryonic Explants to Study Cardiac