Two-Hybrid Systems

Part I. General Methods

- The Two-Hybrid System: A Personal View Stanley Fields and Paul L. Bartel

- Growth and Maintenance of Yeast Lawrence W. Bergman

- Media Formulations for Various Two-Hybrid Systems Michael Saghbini, Denise Hoekstra, and Jim Gautsch

- Yeast Two-Hybrid Vectors and Strains Philip James

- High-Efficiency Transformation of Plasmid DNA into Yeast Robin A. Woods and R. Daniel Gietz

- Qualitative and Quantitative Assessment of Interactions Monica M. Montano

- Strategies for Rescuing Plasmid DNA from Yeast Two-Hybrid Colonies, Alyson Byrd and René St-Arnaud

Part II. False Positives

- Two-Hybrid System and False Positives: Approaches to Detection and Elimination Ilya G. Serebriiskii and Erica A. Golemis

- Confirming Yeast Two-Hybrid Protein Interactions Using In Vitro Glutathione-S-Transferase Pulldowns Dennis M. Kraichely and Paul N. MacDonald

- Two-Hybrid Interactions Confirmed by Coimmunoprecipitation of Epitope-Tagged Clones Louie Naumovski

Part III. Applications

- Smad Interactors in Bone Morphogenetic Protein Signaling Xiangli Yang and Xu Cao

- Protein Interactions Important in Eukaryotic Translation Initiation Katsura Asano and Alan G. Hinnebusch

- Steroid Receptor and Ligand-Dependent Interaction with Coactivator Proteins Sergio A. Oñate

- Interaction of Cellular Apoptosis Regulating Proteins with Adenovirus Anti-Apoptosis Protein E1B-19K Thirugnana Subramanian and G. Chinnadurai

Part IV. Alternative Strategies

- Mammalian Two-Hybrid Assays: Analyzing Protein-Protein Interactions in the Transforming Growth Factor-_ Signaling Pathway Xin-Hua Feng and Rik Derynck

- One-Hybrid Systems for Detecting Protein-DNA Interactions Mary Kate Alexander, Brenda D. Bourns, and Virginia A. Zakian

- The Split-Hybrid System: Uncoding Multiprotein Networks and Defining Mutations That Affect Protein Interactions Phyllis S. Goldman, Anthony J. DeMaggio, Richard H. Goodman, and Merl F.

"The utility of the technique makes this book worthwhile since all the information necessary to get started is included. Anyone should be able to start using the two-hybrid system in their lab using this book as a guide. The book is written for all levels of expertise from student to senior investigator. Each chapter is written by an expert in that particular area. The protocols are detailed and easy to follow and even the preparation of standard buffers is described. A great deal of information on basic yeast techniques is provided for investigators who have not previously worked with yeast. Each chapter contains numerous notes and insights on what to keep an eye out for and potential problems. This would be an excellent addition to any laboratory considering starting the two-hybrid system. The first chapter on the origins of the method is a must read for everyone, especially students." -- 98 Score, 5 stars, Doody's Health Science Book Review Journal

"The stated goal of the volume is "to introduce the yeast two-hybrid systems to students, research assistants, research associates, and other more senior investigators considering this as a new approach." This goal is well achieved as the volume is easy to read and comprehensive.... the first section of this volume provides in depth detailing of yeast protocols, including several very useful trouble shooting solutions and tips well beyond the scope of those found in the instruction manuals accompanying commercial kits....this book is a worthwhile investment to ensure that the most is gained from your two-hybrid experiments and thus I would recommend it." -- Molecular Biotechnology

"This current volume is a practical, laboratory manual with detailed methods and trouble-shooting tips for the yeast two-hybrid approach. The volume includes a brief historical overview, with chapters addressing yeast husbandry and media preparation and yeast genetic modification. The manual lso includes detailed accounts of how to

"The utility of the technique makes this book worthwhile since all the information necessary to get started is included. Anyone should be able to start using the two-hybrid system in their lab using this book as a guide. The book is written for all levels of expertise from student to senior investigator. Each chapter is written by an expert in that particular area. The protocols are detailed and easy to follow and even the preparation of standard buffers is described. A great deal of information on basic yeast techniques is provided for investigators who have not previously worked with yeast. Each chapter contains numerous notes and insights on what to keep an eye out for and potential problems. This would be an excellent addition to any laboratory considering starting the two-hybrid system. The first chapter on the origins of the method is a must read for everyone, especially students."-98 Score, 5 stars, Doody's Health Science Book Review Journal

"The stated goal of the volume is "to introduce the yeast two-hybrid systems to students, research assistants, research associates, and other more senior investigators considering this as a new approach." This goal is well achieved as the volume is easy to read and comprehensive.... the first section of this volume provides in depth detailing of yeast protocols, including several very useful trouble shooting solutions and tips well beyond the scope of those found in the instruction manuals accompanying commercial kits....this book is a worthwhile investment to ensure that the most is gained from your two-hybrid experiments and thus I would recommend it." - Molecular Biotechnology

"This current volume is a practical, laboratory manual with detailed methods and trouble-shooting tips for the yeast two-hybrid approach. The volume includes a brief historical overview, with chapters addressing yeast husbandry and media preparation and yeast genetic modification. The manual lso includes detailed accountsof how to