Target Identification and Validation in Drug Discovery

Part I: Identification of Novel Targets Using High Throughput Screening Assays

1. Using Functional Genetics in Haploid Cells for Drug Target Identification

Jennifer C. Volz, Nicole Schuller, and Ulrich Elling

2. Unbiased Forward Genetic Screening with Chemical Mutagenesis to Uncover Drug-Target Interactions

Moritz Horn, Franziska Metge, and Martin S. Denzel

3. Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development

Konstantinos Drosopoulos and Spiros Linardopoulos

4. CellProfiler and KNIME: Open Source Tools for High Content Screening

Martin Stöter, Antje Janosch, Rico Barsacchi, and Marc Bickle

Part II: Drug Target Profiling and Validation

5. In Silico Target Druggability Assessment: From Structural to Systemic Approaches

Jean-Yves Trosset and Christian Cavé

6. In Silico Drug-Target Profiling

Jean-Yves Trosset and Christian Cavé

7. Locus-Specific Knock-In of a Degradable Tag for Target Validation Studies

Matthias Brand and Georg E. Winter

8. Expression of Human VH Single Domains as Fc-Fusions in Mammalian Cells

Mahmoud Abdelatti, Peter Schofield, and Daniel Christ

Part III: Three Dimensional Cell Culture Techniques Mimicking the Tumor Microenvironment In Vitro

9. The NeuroSphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs) Rossella Galli

10. 3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell Infiltration

Annika Osswald, Viola Hedrich, and Wolfgang Sommergruber

11. Establishment and Analysis of a 3D Co-Culture Spheroid Model of Pancreatic Adenocarcinoma for Application in Drug Discovery

Julia C. Meier-Hubberten and Michael P. Sanderson

Part IV: Animal Models for the Study of Gene Function In Vivo

12. In Vivo Pharmacology Models for Cancer Target Research Dawei