Stem Cell Renewal and Cell-Cell Communication
Methods and Protocols
(Sprache: Englisch)
This volume examines cell-cell interactions and stem cell renewal, two topics that are now inexorably linked as science strives to understand the stem cell niche and its function. Gathering a number of representative protocols, this detailed collection...
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Klappentext zu „Stem Cell Renewal and Cell-Cell Communication “
This volume examines cell-cell interactions and stem cell renewal, two topics that are now inexorably linked as science strives to understand the stem cell niche and its function. Gathering a number of representative protocols, this detailed collection promises to provide readers with approaches for studying these complimentary aspects of stem cells. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Practical and reliable, Stem Cell Renewal and Cell-Cell Communication: Methods and Protocols will aid researchers in using these methods to advance their own studies.
Inhaltsverzeichnis zu „Stem Cell Renewal and Cell-Cell Communication “
1. Laboratory Control and Basic Culture Protocols for Stem Cell Self-RenewalJeong Mook Lim, Yoon Mi Jo, and Ji Yeon Ahn
2. Advanced Fixation for Transmission Electron Microscopy Unveils Special Extracellular Matrix Within the Renal Stem/Progenitor Cell Niche
Will W. Minuth and Lucia Denk
3. Multiplex Immunoassays for Quantification of Cytokines, Growth Factors, and Other Proteins in Stem Cell Communication
Ivona Valekova, Helena Kupcova Skalnikova, Karla Jarkovska, Jan Motlik, and Hana Kovarova
4. Porous Membrane Culture Method for Expansion of Human Pluripotent Stem Cells
Jin-Su Kim, Seung-Taeh Hwang, and Soo-Hong Lee
5. Tuning Differentiation Signals for Efficient Propagation and In Vitro Validation of Rat Embryonic Stem Cell Cultures
Stephen Meek, Linda Sutherland, and Tom Burdon
6. Generation, Expansion, and Differentiation of Human Pluripotent Stem Cell (hPSC) Derived Neural Progenitor Cells (NPCs)
David A. Brafman
7. Isolation, Long-Term Expansion, and Differentiation of Murine Neural Stem Cells
Alexandra Bizy and Sacri R. Ferrón
8. Generation, Expansion, and Differentiation of Cardiovascular Progenitor Cells from Human Pluripotent Stem Cells
Nan Cao, He Liang, and Huang-Tian Yang
9. A Practical Guide for the Isolation and Maintenance of Stem Cells from Tendon
Pauline Po Yee Lui
10. A Human Colonic Crypt Culture System to Study Regulation of Stem Cell-Driven Tissue Renewal and Physiological Function
Alyson Parris and Mark R. Williams
11. Ca2+ Handling in Mouse Embryonic Stem Cell Derived Cardiomyocytes
Wenjie Wei and Jianbo Yue
12. Potential Application of Extracellular Vesicles of Human Adipose Tissue-Derived Mesenchymal Stem Cells in Alzheimer's Disease Therapeutics
Takeshi Katsuda, Katsuyuki Oki, and Takahiro Ochiya
13. Application of Fluid Mechanical Force to Embryonic Sources of Hemogenic Endothelium and Hematopoietic Stem Cells
Nan Li, Miguel F. Diaz, and Pamela L. Wenzel
14.
... mehr
Electrophysiological Recordings from Neuroepithelial Stem Cells
Masayuki Yamashita
15. In Vivo Stem Cell Transplantation Using Reduced Cell Numbers
Takeo W. Tsutsui
Masayuki Yamashita
15. In Vivo Stem Cell Transplantation Using Reduced Cell Numbers
Takeo W. Tsutsui
... weniger
Bibliographische Angaben
- 2015, 2015, X, 210 Seiten, 24 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Kursad Turksen
- Verlag: Springer, Berlin
- ISBN-10: 149392589X
- ISBN-13: 9781493925896
- Erscheinungsdatum: 20.05.2015
Sprache:
Englisch
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