Sirtuins

Introduction: Sirtuins in Aging and Diseases.- Sirtuins in Yeast: Phenotypes and Tools.- Studying the C. elegans Sirtuins.- Genetic and Biochemical Tools for Investigating Sirtuin Function in Drosophila melanogaster.- Generating Mammalian Sirtuin Tools for Protein-Interaction Analysis.- Mass Spectrometry-Based Detection of Protein Acetylation.- SILAC-Based Quantification of Sirt1-Responsive Lysine Acetylome.- Targeted Quantitation of Acetylated Lysine Peptides by Selected Reaction Monitoring Mass Spectrometry.- Identification of Deacetylase Substrates with the Biotin Switch Approach.- Assaying Chromatin Sirtuins.- Measurement of Sirtuin Enzyme Activity Using a Substrate-Agnostic Fluorometric Nicotinamide Assay.- Detecting Sirtuin-Catalyzed Deacylation Reactions Using 32P-Labeled NAD and Thin-Layer Chromatography.- Chemical Acetylation And Deacetylation.- Accurate Measurement of Nicotinamide Adenine Dinucleotide (NAD+) with High-Performance Liquid Chromatography.- In Vivo Measurement of the Acetylation State of Sirtuin Substrates as a Proxy for Sirtuin Activity.- Oxygen Flux Analysis to Understand the Biological Function of Sirtuins.- The Emerging Links Between Sirtuins and Autophagy.- Methods to Study the Role of Sirtuins in Genome Stability.- Circadian Measurements of Sirtuin Biology.- Utilizing Calorie Restriction to Evaluate the Role of Sirtuins in Healthspan and Lifespan of Mice.