Single Particle Tracking and Single Molecule Energy Transfer

PrefacePART I: Single-Particle Imaging and TrackingTHREE DIMENSIONAL PARTICLE TRACKING IN A LASER SCANNING FLUORESCENCE MICROSCOPEIntroductionImage-Based Single-Particle Tracking MethodsAdvanced Fluorescence Microscopy Techniques for Single-Particle TrackingTwo-Photon Excitation Microscopy3-D Tracking in Image-Based SPT Approaches3-D Tracking in Laser Scanning MicroscopesInstrumentationBackground NoiseSimultaneous Two-Particle TrackingApplication: Chromatin Dynamics in Interphase CellsConclusionsTHE TRACKING OF INDIVIDUAL MOLECULES IN CELLS AND TISSUESIntroductionSingle-Molecule and Single-Particle LocalizationPositional AccuracyTrackingTrajectory AnalysisApplicationsConclusionsMESSENGER RNA TRAFFICKING IN LIVING CELLSIntranuclear Structure and DynamicsFCS and FRAP Studies of Nuclear mRNP MobilitySingle-Particle Tracking of mRNA MoleculesSingle-Particle Tracking of Specific, Native mRNPsIn Vivo Labeling of Native BR2 mRNPsOutlook: Light Sheet-Based Single-Molecule MicroscopyQUANTUM DOTS: INORGANIC FLUORESCENT PROBES FOR SINGLE-MOLECULE TRACKING EXPERIMENTS IN LIVE CELLSIntroductionFluorescent Labels for Single-Molecule Tracking in CellsOptical Properties of Colloidal Quantum DotsSynthesis of Colloidal Fluorescent Quantum DotsSurface Chemistry for the Water-Solubilization of Quantum DotsInterfacing Quantum Dots with BiologySingle Quantum Dot Tracking Experiments in Live CellsConclusions and PerspectivesPART II: Energy Transfer on the NanoscaleSINGLE-PAIR FRET: AN OVERVIEW WITH RECENT APPLICATIONS AND FUTURE PERSPECTIVESIntroductionPrinciples of FRETspFRET in SolutionspFRET on Immobilized MoleculesFuture ProspectsALTERNATING-LASER EXCITATION AND PULSED-INTERLEAVED EXCITATION OF SINGLE MOLECULESIntroductionALEX: The Principles of OperationMyALEXNanosecond-ALEX/Pulsed Interleaved Excitation (PIE)msALEXThree-Color ALEXConclusions and OutlookUNRAVELING THE DYNAMICS BRDIGING PROTEIN STRUCTURE AND FUNCTION ONE MOLECULE AT A TIMEIntroductionConverting Chemical Energy to

Christoph Bräuchle is Professor at the Ludwig-Maximilian-University (LMU) Munich. After his PhD, he spent one year as a postdoc at IBM in San José, California, USA. His research focuses on imaging, spectroscopy and manipulation of single molecules in bio- and nano-sciences. Besides numerous publications in international journals, Prof. Bräuchle has won several honors, including the prestigious Philip Morris Research award and the Karl Heinz Beckurts Prize 2002. He also is a member of the Academia Europaea.Don C. Lamb is Professor for Biophysical Chemistry at the LMU Munich.He received his PhD from the University of Illinois at Urbana-Champaign and was a research fellow at the Harvard Medical School, an Alexander von Humboldt Research Fellow at the TU Munich, a member of the Laboratory for Fluorescence Dynamics at the University of Illinois at Urbana-Champaign and a visiting scientist at the University of Ulm. His research centers around ultra-sensitive fluorescence methods, single molecule studies, protein function and dynamics, fluorescence fluctuation spectroscopies, live-cell imaging, single particle tracking and single virus tracing. Jens Michaelis is an Assistant Professor at the LMU Munich. After receiving his PhD in physics in 2000, he spent several years as a postdoc at the University of California, Berkley, focusing on single-molecule studies of molecular motors. His research interests are the molecular mechanisms underlying the biological activity of proteins and mechanical properties of polymer molecules. In 2007 he was awarded the Römer Prize from the LMU for his habilitation work.