Research Methods in Neurochemistry
Volume 1
(Sprache: Englisch)
On picking up this first volume of a new series of books the reader may ask the two questions: (a) why research methods? and (b) why in neurochem istry? The answers to these questions are easy - they more than justify the volumes to come and show the strong...
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Klappentext zu „Research Methods in Neurochemistry “
On picking up this first volume of a new series of books the reader may ask the two questions: (a) why research methods? and (b) why in neurochem istry? The answers to these questions are easy - they more than justify the volumes to come and show the strong need for their existence. It is customary to think of methods as a necessary but unexciting means to an end - to relegate advances in methodology to a minor role in the creative, original portion of advances in science. This is not the case; the pace-setting function of methodology is well illustrated in most areas of neurobiology. To formulate our questions to Nature (which is the essence of experimental design), methodology is needed; to get answers to our ques tions we have to devise yet new methods. The chapters of the present volume fully illustrate how the development of a new method can cut a new path how it can open new fields, just as the microscope founded histology. Heter ogeneity of structures presents a formidable challenge for methodology in the nervous system, yet methods for separating the structures are essential if we ever want to decipher the enigma of functional contribution of the ele ments to the whole. The problem is not only physical separation-clearly methods are essential to study complex structures in situ.
Inhaltsverzeichnis zu „Research Methods in Neurochemistry “
Section I. Ultrastructure and Fragmentation of Neural Tissues.- 1 Nervous System Cell Preparations: Microdissection and Micromanipulation.- 2 The Bulk Separation of Neuroglia and Neuron Perikarya.- 3 Separation of Myelin Fragments from the Central Nervous System.- 4 Principles for the Optimization of Centrifugation Conditions for Fractionation of Brain Tissue.- 5 Brain Ribosomes.- 6 Isolation of Brain Cell Nuclei.- Section II. Properties of Intact Neural Tissues.- 7 Ventriculocisternal Perfusion as a Technique for Studying Transport and Metabolism within the Brain.- 8 The Estimation of Extracellular Space of Brain Tissue in Vitro.- Section III. Components of Neural Tissues.- 9 Ethanolamine Plasmalogens.- 10 Methods for Separation and Determination of Gangliosides.- 11 Mucopolysaccharides and Glycoproteins.- Section IV. Biologically Active Amines.- 12 Assay of Biogenic Amines and Their Deaminating Enzymes in Animal Tissues.- 13 Enzymes Involved in the Catalysis of Catecholamine Biosynthesis.- 14 Detection and Quantitative Analysis of Some Noncatecholic Primary Aromatic Amines.
Bibliographische Angaben
- 2012, 1972, XXII, 368 Seiten, Maße: 15,2 x 22,9 cm, Kartoniert (TB), Englisch
- Herausgegeben: Neville Marks
- Verlag: Springer, Berlin
- ISBN-10: 1461577500
- ISBN-13: 9781461577508
Sprache:
Englisch
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