Protein NMR Techniques

Table of Contents1. A Novel Bacterial Expression Method with Optimized Parameters for Very High Yield Production of Triple-labeled Proteins2 Isotopic Labeling of Heterologous Proteins in the Yeast Pichia pastoris and Kluyveromyces lactis3. Isotope Labeling in Insect Cells4. Isotope Labeling in Mammalian Cells5. Cell-free Protein Production for NMR Studies6. Cell-free Membrane Protein Expression for Solid-state NMR7. Expression and Purification of Src-family Kinases for Solution NMR Studies8. NMR Studies of Large Protein Systems9. Protein Dynamics by 15N Nuclear Magnetic Relaxation10. Bacterial Production and Solution NMR Studies of a Viral Membrane Ion Channel11. Preparation of the Modular Multi-Domain Protein RPA for Study by NMR Spectroscopy12. NMR Studies of Protein-RNA Interactions13. Preparation and Optimization of Protein-DNA Complexes Suitable for Detailed NMR Studies14. NMR Studies of Protein-ligand Interactions15. In-cell NMR Spectroscopy in Escherichia coli16. Deuterated Peptides and Proteins: Structure and Dynamics Studies by MAS solid-state NMR17. Solid-State NMR Spectroscopy of Protein Complexes18. Synthesis, Purification and Characterization of Single Helix Membrane Peptides and Proteins for NMR Spectroscopy19. Assignment of Backbone Resonances in a Eukaryotic Protein Kinase ¿ ERK220. Electrostatics of Hydrogen Exchange for Analyzing Protein Flexibility21. Fast Protein Backbone NMR Resonance Assignment using the BATCH Strategy22. Comprehensive Automation for NMR Structure Determination of Proteins 23. ARIA for Solution and Solid-state NMR Protein NMR Techniques24. Determining Protein Dynamics from 15N Relaxation Data by using DYNAMICS