Protein Crystallography

1. Expression and Purification of Recombinant Proteins in Escherichia Coli with a His6 or Dual His6-MBP Tag

Sreejith Raran-Kurussi and David S. Waugh

2. Protein Crystallization

Alexander McPherson

3. Advanced Methods of Protein Crystallization

Abel Moreno

4. The 'Sticky Patch' Model of Crystallization and Modification of Proteins for Enhanced Crystallizability

Zygmunt S. Derewenda and Adam Godzik

5. Crystallization of Membrane Proteins: and Overview

Andrii Ishchenko, Enrique Abola, and Vadim Cherezov

6. Locating and Visualizing Crystals for X-ray Diffraction Experiments

Michael Becker, David J. Kissick, and Craig M. Ogata

7. Collection of X-ray Diffraction Data from Macromolecular Crystals

Zbigniew Dauter

8. Identifying and Overcoming Crystal Pathologies: Disorder and Twinning

Michael C. Thompson

9. Applications of X-ray Micro-beam for Data Collection Ruslan Sanishvili and Robert F. Fischetti

10. Serial Synchrotron X-ray Crystallography (SSX)

Kay Diederichs and Meitian Wang

11. Time-Resolved Macromolecular Crystallography at Modern X-ray Sources

Marius Schmidt

12. Structure Determination Using X-ray Free-electron Laser Pulses

Henry N. Chapman

13. Processing of XFEL Data

Thomas A. White

14. Many Ways to Derivatize Macromolecules and their Crystals for Phasing

Miroslawa Dauter and Zbigniew Dauter

15. Experimental Phasing: Substructure Solution and Density Modification as Implemented in SHELX Andrea Thorn

16. Contemporary Use of Anomalous Diffraction in Biomolecular Structure Analysis

Qun Liu and Wayne A. Hendrickson

17. Long-Wavelength X-ray Diffraction and its Applications in Macromolecular Crystallography

Manfred S. Weiss

18. Acknowledging Errors: Advanced Molecular Replacement