Plasmodesmata

Plasmodesmata Structural Components and Their Role in Signaling and Plant Development.- Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses.- Plasmodesmata Ultrastructure Determination Using Electron Tomography.- Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata.- Serial Block Electron Microscopy to Study Plasmodesmata in the Vasculature of Arabidopsis thaliana Roots.- Focused Ion Beam-Scanning Electron Microscopy for Investigating Plasmodesmal Densities.- Measuring Plasmodesmata Density on Cell Interfaces of Monocot Leaves Using 3D Immunolocalization and Scanning Electron Microscopy.- Super-Resolution Imaging of Plasmodesmata Using 3D-Structured Illumination Microscopy.- In Vivo Aniline Blue Staining and Semi-Automated Quantification of Callose Deposition at Plasmodesmata.- Immunofluorescence Detection of Callose in Plant Tissue Sections.- Callose Detection and Quantification at Plasmodesmata in Bryophytes.-Isolation of Plasmodesmata Membranes for Lipidomic and Proteomic Analysis.- Methods for Detection of Protein Interactions with Plasmodesmata-Localized Reticulons.- Studying Protein-Protein Interactions at Plasmodesmata by Measuring Förster Resonance Energy Transfer.- Quantifying the Organization and Dynamics of the Plant Plasma-Membrane across Scales Using Light Microscopy.- Using Steady-State Fluorescence Anisotropy to Study Protein Clustering.- Quantification of Cell-to-Cell Connectivity Using Particle Bombardment.- Investigating Plasmodesmata Function in Arabidopsis thaliana Using a Low-Pressure Bombardment System and GFP Movement Assay.- Quantifying Plasmodesmatal Transport with an Improved GFP Movement Assay.- An Arabidopsis Callus Grafting Method to Test Cell-to-Cell Mobility of Proteins.- Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP.- Tracking Intercellular Movement of Fluorescent Proteins in Bryophytes.- Virus Genome-Based Reporter for