Plant Signal Transduction

Suppressor Screens in Arabidopsis.- Quantitative Reverse Transcription-qPCR-Based Gene Expression Analysis in Plants.- DNA-Binding Factor Target Identification by Chromatin Immunoprecipitation (ChIP) in Plants.- Identification of Genes Responsible for Natural Variation in Volatile Content Using Next Generation Sequencing Technology.- Micro-Tom Tomato as an Alternative Plant Model System: Mutant Collection and Efficient Transformation.- Culture of the Tomato Micro-Tom Cultivar in Greenhouse.- Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry.- A Simplified and Rapid Method for the Isolation and Transfection of Arabidopsis Leaf Mesophyll Protoplasts for Large-Scale Applications.- Preparation of Epidermal Peels and Guard Cell Protoplasts for Cellular, Electrophysiological, and -Omics Assays of Guard Cell Function.- Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells.- Immunoprecipitation of Plasma Membrane Receptor-Like Kinases for Identification of Phosphorylation Sites and Associated Proteins.- Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions.- Cautions in Measuring In Vivo Interactions Using FRET and BiFC in Nicotiana benthamiana.- Analysis of Protein-Lipid Interactions Using Purified C2 Domains.- Assessing Kinase Activity in Plants with In-Gel Kinase Assays.- Analyses of Plant UDP-Dependent Glycosyltransferases to Identify Their Volatile Substrates Using Recombinant Proteins.- Competitive Index: Mixed Infection-Based Virulence Assays for Genetic Analysis in Pseudomonas syringae-Plant Interactions.- Simplified Assays for Evaluation of Resistance to Alternaria brassicicola and Turnip Mosaic Virus.- Simultaneous Determination of Plant Hormones by GC-TOF-MS.