Plant Immunity

Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression.- Fragment Complementation and Co-Immunoprecipitation Assays for Understanding R Protein Structure and Function.- Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag.- Biochemical Purification of Native Immune Protein Complexes.- Chromatin Immunoprecipitation (ChIP) to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity.- Dose Response to and Systemic Movement of Dexamethasone in the GVG Inducible Transgene System in Arabidopsis.- Quantifying Alternatively Spliced mRNA via Capillary Electrophoresis.- Constructing Haustorium-Specific cDNA Libraries from Rust Fungi.- Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic Nematodes.- Construction of Pseudomonas syringae pv. tomato DC3000 Mutant and Polymutant Strains.- A Straightforward Protocol for Electro-Transformation of Phytophthora capsici Zoospores.- Propagation, Storage, and Assays with Hyaloperonospora arabidopsidis, a Model Oomycete Pathogen of Arabidopsis.- Assaying Effector Function In planta Using Double-Barreled Particle Bombardment.- Assays for Effector-Mediated Suppression of Programmed Cell Death in Yeast.- Purification of Effector-Target Protein Complexes via Transient Expression in N. benthamiana.- Imaging Fluorescently-Tagged Phytophthora Effector Proteins Inside Infected Plant Tissue.- Immunolocalization of Pathogen Effectors.- Laser Capture Microdissection of Nematode Feeding Cells.- Laser Microdissection of Plant-Fungus Interaction Sites and Isolation of RNA for Downstream Expression Profiling.- Global Expression Profiling of RNA from Laser Microdissected Cells at Fungal-Plant Interaction Sites.- Visualizing Cellular Dynamics in Plant-Microbe Interactions Using Fluorescent-Tagged Proteins.

From the reviews:

"This volume ... describes newly developed or optimised techniques for understanding the immune response of plants and the ways in which pathogens, including the true fungi, oomycetes, nematodes and bacteria, are able to subvert it. ... Where appropriate, methods are well illustrated with diagrams, photographs of equipment and expected results. In summary, this is a timely addition to this series and will be of immediate interest and use to all those working in this field." (Andrew James, Annals of Botany, Vol. 111 (1), January, 2013)

From the reviews:

"This volume ... describes newly developed or optimised techniques for understanding the immune response of plants and the ways in which pathogens, including the true fungi, oomycetes, nematodes and bacteria, are able to subvert it. ... Where appropriate, methods are well illustrated with diagrams, photographs of equipment and expected results. In summary, this is a timely addition to this series and will be of immediate interest and use to all those working in this field." (Andrew James, Annals of Botany, Vol. 111 (1), January, 2013)