Plant Cell Morphogenesis

Essential Methods of Plant Sample Preparation for Light Microscopy.- Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy.- Resin Embedding, Sectioning and Immunocytochemical Analyses of Plant Cell Walls in Hard Tissues.- Automated Microscopy in Forward Genetic Screening of Arabidopsis.- Image Analysis: Basic Procedures for Description of Plant Structures on Anatomical Sections.- Identifying Subcellular Protein Localization with Fluorescent Protein Fusions After Transient Expression in Onion Epidermal Cells.- Visualizing and Quantifying the in vivo Structure and Dynamics of the Arabidopsis Cortical Cytoskeleton using CLSM and VAEM.- Sequential Replicas for In Vivo Imaging of Growing Organ Surfaces.- Time-lapse Imaging of Developing Meristems using Confocal Laser Scanning Microscope.- Quantifying Cell Shape and Gene Expression in the Shoot Apical Meristem using MorphoGraphX.- Mechanical Measurements on Living Plant Cells by Micro-indentation with Cellular Force Microscopy.- High-pressure Freezing and Low-temperature Processing of Plant Tissue Samples for Electron Microscopy.- Reconstructing Plant Cells in 3D by Serial Section Electron Tomography.- Imaging Plant Nuclei and Membrane-associated Cytoskeleton by Field Emission Scanning Electron Microscopy.- Immuno-gold Labeling of Resin-embedded Electron Microscopical Sections.- Live Cell Imaging of Arabidopsis Root Hairs.- Morphological Analysis of Cell Growth Mutants in Physcomitrella.- Plant Cell Lines in Cell Morphogenesis Research.- Antisense Oligodeoxynucleotide-mediated Gene Knock-down in Pollen Tubes.- Lab-on-a-Chip for Studying Growing Pollen Tubes.- Laser Microdissection of Plant Cells.- Optical Trapping in Plant Cells.- Heterologous Expression in Budding Yeast as a Tool for Studying the Plant Cell Morphogenesis Machinery.

From the book reviews:"A compendium of procedures and techniques for investigating the structure and shape of plant tissues and cells in a developmental context. ... I can certainly rate this book very useful for the scientific community, as it contains many valuable new techniques and inside knowledge. Furthermore, it could inspire researchers to use new technical approaches for their research topics and discuss these with the expert authors of these articles." (Kai R. Konrad, Journal of Plant Physiology, Vol. 171, 2014)