Microglia

A Brief Overview of Multi-Talented Microglia.- Cell Culturing of Human and Murine Microglia Cell Lines.- Microglia Isolation from Adult Mouse Brain.- Preparation of Primary Microglia Cultures from Postnatal Mouse and Rat Brains.- Isolation of Murine Postnatal Brain Microglia for Phenotypic Characterization Using Magnetic Cell Separation Technology.- Isolation and Culture of Adult Human Microglia within Mixed Glial Cultures for Functional Experimentation and High Content Analysis.- Depletion of Microglia from Primary Cellular Cultures.- Lentiviral Transduction of Cultured Microglia.- Microglial Activation: Measurement of Cytokines by Flow Cytometry.- In situ Hybridization of Cytokine mRNA using Alkaline Phosphatase Labelled Oligodeoxynucleotide Probes.- Use of Meso-scale DiscoveryTM to Examine Cytokinecontentin Microglia Cell Supernatant.- Analysis of Microglial Production of Reactive Oxygen and Nitrogen Species.- Quantification of Active Caspase-3 and Active Caspase-8 in Microglia Cells.- Quantification of Microglial Phagocytosis by a Flow Cytometer-based Assay.- Quantification of Microglial Proliferation and Apoptosis by Flow Cytometry.- Fluorescence Imaging of Intracellular Ca2+, Na+ and H+ in Cultured Microglia.- Patch Clamp Protocols to Study Ion Channel Activity in Microglia.- Studying M1 and M2 States in Adult Microglia.- Isolating, Culturing, and Polarizing Primary Human Adult and Fetal Microglia.- Understanding Microglia-neuron Cross-talk: Relevance of the Microglia-neuron Co-cultures.- Preparation of Rodent Primary Cultures for Neuron-Glia, Mixed Glia, Enriched Microglia, and Reconstituted Cultures with Microglia.- Microglia Detection by Enzymatic Histochemistry.- Tomato Lectin Histochemistry for Microglial Visulization.- Immunohistochemical Detection of Microglia.- Intrathecal Infusion of Microglia Cells.- Intracranial Injection of LPS in Rat as Animal Model of Neuroinflammation.- Analyses ofMicroglia Effector Function Using CX3CR1-GFP Knock In