Mammalian Synthetic Promoters

Initial Considerations before Designing a Promoter Construct.- Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay.- Chromatin Immunoprecipitation and Quantitative Real-time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites within a Promoter.- Secreted Reporters for Monitoring Multiple Promoter Function.- Bioluminescence Monitoring Of Promoter Activity In Vitro and In Vivo.- Monitoring Promoter Activity by Flow Cytometry.- Functional Screening of Core Promoter Activity.- Bioinformatically Informed Design of Synthetic Mammalian Promoters.- Synthetic Tumor Specific Promoters for Transcriptional Regulation of Viral Replication.- Constructing Strong Cell-Type-Specific Promoters through Informed Design.- PCR Assembly of Synthetic Promoters.- The Tetracycline Responsive System.- Light-responsive Promoters.- A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation.- Promoter Activation with Electromagnetism.- Application of Synthetic Tumor Specific Promoters Responsive to the Tumor Microenvironment.- A Modular Approach to Building Complex Synthetic Circuits.- Computational Sequence Design with R2oDNA Designer.- Design of Synthetic Promoters for Gene Circuits in Mammalian Cells.