Malaria Immunology

proteins.- Analysis of antibody reactivity to malaria antigens by microsphere-based multiplex immunoassay.- High-throughput BioPlex assay for the study of functionally active Plasmodium falciparum antigens that are expressed on the surface of infected erythrocytes.- Protein microarrays as a tool to analyze antibody responses to variant surface antigens expressed on the surface of Plasmodium falciparum-infected erythrocytes.- Protocol for differential biopanning of Plasmodium falciparum phage display cDNA library to identify parasite targets of protective antibodies.- Affinity purification of PfEMP1-specific antibodies from human blood.- Production of anti-PfEMP1 polyclonal antisera in rats and mice.- Production of PfEMP1-specific mouse monoclonal antibodie.- Production of PfEMP1-specific human monoclonal antibodies from naturally immune individuals.- Visualization of infected red blood cell surface antigens by fluorescence microscop.- Analysis by flow cytometry of 2 macroglobulin and non-immune IgM-binding to Plasmodium falciparum-infected erythrocytes.- An improved method for assessing antigen presentation on the surface of Plasmodium falciparum-infected erythrocytes by immuno-electron microscopy.- Assessing antigen presentation on the surface of Plasmodium falciparum-infected erythrocytes by photoactivated localization microscopy (PALM).- Surface plasmon resonance analysis of PfEMP1 interaction with receptors.- Analysis of antibody neutralization of PfEMP1 binding by competition ELISA.- Measuring rosetting inhibition in Plasmodium falciparum parasites using a flow cytometry-based assay.- Bridging and clumping: investigating platelet interactions with Plasmodium falciparum-infected red blood cells and endothelial cells in cerebral malaria.- Assay of static adhesion of Plasmodium falciparum-infected erythrocytes to cells, including inhibition of the adhesion.- Static adhesion of Plasmodium falciparum-infected erythrocytes to purified and recombinant receptors.- Selecting Plasmodium falciparum-infected erythrocytes for adhesion to recombinant receptors under flow conditions.- Chip-based assay of adhesion of Plasmodium falciparum-infected erythrocytes to cells under flow.- Binding of Plasmodium falciparum-infected red blood cells to engineered 3D microvessels.- 3D organoid assay of the impact of Plasmodium falciparum-infected erythrocyte adhesion on the blood-brain barrier.- Receptor-functionalized lipid membranes as biomimetic surfaces for adhesion of Plasmodium falciparum-infected erythrocytes.- Antibody-dependent THP-1 cell-mediated phagocytosis of Plasmodium falciparum-infected erythrocytes.- Assaying interactions between neutrophils and Plasmodium falciparum-infected red blood cells.- Natural killer cell antibody dependent cellular cytotoxicity (ADCC) activity against Plasmodium falciparum-infected red blood cells.- Assessing PfGARP-mediated apoptosis of blood-stage Plasmodium falciparum parasites.- Enzyme-linked immunosorbent assay for activation of the classical complement pathway by Plasmodium falciparum-infected erythrocyte surface antigen-specific antibodies.- Flow cytometry assay of Plasmodium falciparum-specific B-cell proportions.- Assessment of Plasmodium falciparum antigen-specific B cells.- Whole blood dendritic cell cytokine production assay.- Measuring the impact of malaria on the living human retina.- Ocular pathology of cerebral malaria.- Investigating interactions between endothelial cells and parasitised red blood cells in skin and subcutaneous tissue.