Epitope Mapping Protocols

An Introduction to Epitope Mapping.- Epitope Mapping of Antibody-Antigen Interactions with X-Ray Crystallography.- Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical Considerations.- Label-Free Cell-Based Assay for Characterization of Biomolecules and Receptors.- Probing Antibody Binding Sites on G Protein-Coupled Receptors Using Genetically-Encoded Photo-Activatable Cross-Linkers.- Rapid Mapping of Protein Binding Sites and Conformational Epitopes by Coupling Yeast Surface Display to Chemical Labeling and Deep Sequencing.- Epitope Mapping Using Yeast Display and Next Generation Sequencing.- B-Cell Epitope Mapping Using a Library of Overlapping Synthetic Peptides in an Enzyme-Linked Immunosorbent Assay.- Array-In-Well Epitope Mapping of Phage-Displayed Antibodies.- Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries.- Discontinuous Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Folded Domains.- Kinetic Analysis and Epitope Binning Using Surface Plasmon Resonance.- Epitope Binning of Human Monoclonal Antibodies in Classical Sandwich and In-Tandem Orientation Using the Octet System Based on Biolayer Interferometry.- Antibody Specificity Profiling Using Protein Microarrays.- High-Density Antigen Microarrays for Assessment of Antibody Selectivity and Off-Target Binding.- Multiplexed Antigen Bead Arrays for Assessment of Antibody Selectivity and Epitope Mapping.- Antibody Biomarker Discovery Via In Vitro Evolution of Peptides.- Bioinformatics Tools for the Prediction of T Cell Epitopes.