Epigenetic Reprogramming During Mouse Embryogenesis

Ex Vivo Culture for Preimplantation Mouse Embryo to Analyse Pluripotency.- Microfabricated Device for High Resolution Imaging of Preimplantation Embryos.- In Vitro Culture of Mouse Blastocyst to the Egg Cylinder Stage via Mural Trophectoderm Excision.- Spatially Organized Differentiation of Mouse Pluripotent Stem Cells on Micropatterned Surfaces.- Mouse Primordial Germ Cells: In Vitro Culture and Conversion to Pluripotent Stem Cell Lines.- Generation of Primordial Germ Cell-Like Cells in Small and Large Scales.- Genome-Scale CRISPR Screening for Regulators of Cell Fate Transitions.- Somatic Reprogramming by Nuclear Transfer.- Targeted Transgenic Mice using CRISPR/Cas9 Technology.- Whole-Mount Immunofluorescence Staining of Early Mouse Embryos.- Investigating the Inner Cell Mass of the Mouse Blastocyst by Combined Immunofluorescence Staining and RNA Fluorescent In Situ Hybridization.- Mapping of Chromosome Territories by 3D-Chromosome Painting during Early Mouse Development.- Deciphering the Early Mouse Embryo Transcriptome by Low-Input RNA-seq.- Studying DNA Methylation Genome-Wide by Bisulfite Sequencing from Low Amounts of DNA in Mammals.- Profiling DNA Methylation Genome-Wide in Single Cells.- Tracking Histone Modifications in Embryos and Low Input Samples using Ultrasensitive STAR ChIP-seq.- Chromatin Profiling in Mouse Embryonic Germ Cells by CUT&RUN.- DamID to Map Genome-Protein Interactions in Pre-Implantation Mouse Embryos.- Understanding Chromosome Structure during Early Mouse Development by Single-Cell Hi-C Analysis.- Bioinformatic Analysis of Single Cell Hi-C Data from Early Mouse Embryo.