Cereal Genomics

DNA Extraction from Vegetative Tissue for Next Generation Sequencing .- DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples) .- RNA Extraction from Cereal Vegetative Tissue.- RNA Extraction from Developing or Mature Wheat Seeds.- cDNA Library Preparation.- Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants.- The Polymerase Chain Reaction (PCR) - General Methods.- Mutation and Mutation Screening.- The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression.- Cloning of DNA Fragments: Ligation Reactions in Agarose Gel.- Rapid Cloning of Genes and Promoters for Functional Analyses.- Genome Walking.- Functional Analysis by Protein Biochemistry.- Genomic Southern Blot analysis.- Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs.- Protein Blotting-Protocol for Beginners.- Genetic Transformation of Wheat via Particle Bombardment.- Sorghum Genetic Transformation by Particle Bombardment.- Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery.- A Protocol for High-throughput Agrobacterium-mediated Barley Transformation.- Agrobacterium-mediated Transformation- Rice Transformation.- Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos.- A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops.- A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data.