Baculovirus

Expansion and Storage of Insect Cells and Virus Stocks.- Construction of Recombinant Baculoviruses.- A Time and Cost-Effective Pipeline for Expression Screening and Protein Production in Insect Cells Based on the HR-Bac Toolbox to Generate Recombinant Baculoviruses.- Optimized Workflow from Gene to Product.- TOPO Cloning for Efficient Plasmid Construction.- Adaptive Laboratory Evolution to Improve Recombinant Protein Production Using Insect Cells.- RNAi-Mediated Silencing in the Insect Cell-Baculovirus Expression System.- Scarless Baculovirus Genome Editing Using Lambda Red Recombineering in E. coli.- Probing Baculovirus Vector Gene Essentiality for Foreign Gene Expression Using a CRISPR-Cas9 System.- Generation of Complex Protein Structures by Co-Infection with High Quality Recombinant Baculovirus.- Production of Secreted Antibody in Baculovirus Expression Vector System.- Production of Recombinant Viral Antigens Using the Baculovirus-Insect Cell Expression System.- Protein Production at the 100L Scale.- Recombinant AAV Production.- Recombinant AAV Purification.- Extraction of Modified Adeno-Associated Virus-Like Particles Displaying Peptides on Their Surface.- One-Step Purification Strategy for Cowpea Chlorotic Mottle Virus-Like Particles Produced by the IC-BEVS.- Purification Process for a Secreted Protein Produced in Insect Cells.- Functional Baculovirus Particle Quantification via Plaque Assay.- TCID50 Assay: A Simple Method to Determine Baculovirus Titer.- Rapid Two-Day Baculovirus Titering Using Flow Cytometry.- A Microsphere Immunoassay for the Quantitative Detection of Antigens in Cell Culture Supernatant.- Non-Viral Platform for Expression of Recombinant Protein in Insect Cells.- Assembly of Baculovirus Vectors for Multiplexed Prime Editing.- Recombinant Protein Production in Suspension Mammalian Cells Using the BacMam Baculovirus Expression System.